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Nutrient Agar Kit, Includes 50 Sterile Petri Dishes with Lids


Nutrient Agar Kit, Includes 50 Sterile Petri Dishes with Lids


Product description

Nutrient Agar is a microbiological growth medium commonly used for the routine cultivation of non-fastidious bacteria. It is useful because it remains solid even at relatively high temperatures. Also, bacteria grown in nutrient agar grows on the surface, and is clearly visible as small colonies. Kit Includes: *7.5 grams Pancreatic Digest of Gelatin (Peptone G) *4.5 grams Beef Extract *22.5 grams Agar Agar *50, 100mm x 15mm Plastic (polystyrene) Petri Dishes with Lids *50 Sterile Cotton Swabs Each Kit will yield approximately 1500ml (1.5 Liters) of Nutrient Agar Solution which will yield 50, 100mm Petri Dishes. FORMULA: 21.7% Pancreatic Digest of Gelatin (Peptone G) 13% Beef Extract 65.2% Agar Agar SOLUTION INSTRUCTIONS: Step 1: Dissolve 7.5g of Peptone G in 1500ml of Distilled Water Step 2: Dissolve 4.5g Beef Extract in the solution from Step 1 Step 3: Dissolve 22.5g Agar Agar in the solution from Step 2 Step 4: Bring to a Boil amp; Stir Step 5: Autoclave at 121 degrees Celsius for 15 minutes Step 6: Pour into Petri Dishes Step 7: Enjoy amp; Record your Experiments! FREQUENTLY ASKED QUESTIONS (FAQ): Q/ What is an Autoclave amp; What does it do? A/ An autoclave is a device that heats the nutrient agar under pressure in order to sterilize it prior to inoculation. An autoclave is essentially a pressure cooker. You can substitute a household pressure cooker for an autoclave. Many modern household pressure cookers will not reach 250 degrees Fahrenheit at 15PSI so you may have to increase the amount of time you "cook" the nutrient agar to insure sterility is achieved. Q/ Do I have to sterilize the Nutrient Agar? A/ Sterilization is highly recommended but not required. Many would-be scientists have successful experiments without the use of an autoclave and without sterilizing the nutrient agar, after all, the objective is generally to grow mold and / or bacteria which will occur either way.

Nutrient Agar Kit, Includes 50 Sterile Petri Dishes with Lids

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